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Related Experiment Videos

Enzyme replacement for lactose malabsorption using a beta-D-galactosidase.

J A DiPalma1, M S Collins

  • 1University of South Alabama College of Medicine, Division of Gastroenterology, Mobile 36617.

Journal of Clinical Gastroenterology
|June 1, 1989
PubMed
Summary
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Oral beta-D-galactosidase from Aspergillus oryzae effectively reduced lactose malabsorption symptoms like bloating and flatulence. This enzyme supplement, when taken with lactose, significantly lowered breath hydrogen excretion in a dose-dependent manner.

Area of Science:

  • Gastroenterology
  • Enzymology
  • Microbiology

Background:

  • Lactose malabsorption affects a significant portion of the population, leading to digestive discomfort.
  • Enzyme replacement therapy is a potential strategy to alleviate symptoms associated with lactose intolerance.

Purpose of the Study:

  • To evaluate the efficacy of oral beta-D-galactosidase derived from Aspergillus oryzae in reducing symptoms and breath hydrogen excretion in lactose malabsorbers.
  • To determine if the enzyme's effect is dose-dependent.

Main Methods:

  • Ten healthy symptomatic lactose malabsorbers participated in a double-blind, placebo-controlled study.
  • Subjects underwent four lactose challenges over separate days, receiving either placebo or two doses (250mg and 500mg) of beta-D-galactosidase before lactose ingestion.

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  • Breath hydrogen levels and gastrointestinal symptoms were monitored for 8 hours post-challenge.

    • Main Results:

    • Beta-D-galactosidase significantly reduced breath hydrogen excretion in a dose-dependent manner, with the 500mg dose showing a statistically significant reduction.
    • A dose-related decrease in bloating and flatulence was observed (p ≤ 0.05).
    • No significant difference was found in other assessed symptoms like cramps, nausea, pain, or diarrhea.

    Conclusions:

    • Oral beta-D-galactosidase from Aspergillus oryzae is effective in reducing breath hydrogen excretion and specific symptoms of lactose malabsorption.
    • The enzyme demonstrates a dose-dependent effect, suggesting its utility as a therapeutic agent for lactose intolerance.