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Updated: Apr 27, 2026
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The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
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Design and establishment of a vector system that enables production of multifusion proteins and easy purification by
Tabea Marquardt1, Amélie von der Heyde1, Skander Elleuche1
1Technische Universität Hamburg-Harburg, Institut für Technische Mikrobiologie, Kasernenstr. 12, D-21073 Hamburg, Germany.
Journal of Microbiological Methods
|July 16, 2014
Abstract:
The LE (LguI/Eco81I)-cloning procedure allows a step-wise, directional fusion of multiple DNA-fragments into a vector by utilizing two restriction enzymes generating identical non-palindromic overhangs. This strategy was applied to produce heat-stable cellulase-fusion proteins containing up to five single moieties. Terminal affinity tags enable efficient purification using a simple two-step approach.
