Related Experiment Video
Updated: Apr 27, 2026

Pilot In Vitro Study to Assess Cleaning Ability and Effects of Different Decontamination Methods on Implant Surfaces
Published on: November 21, 2025
[Cleanliness of the walls of root canals in teeth with incomplete development of root, rinsed with 2% chlorexidine. A
Introduction:
The aim of the study was to evaluate the cleanliness of the walls of root canals with incomplete development of root, whose lumen was rinsed with 2% chlorhexidine solution during mechanical preparation.
Material And Methods:
Use was made of 8 maxillary premolar teeth with a single root canal and with incomplete development of the root apex (the teeth were removed during the growing stage for orthodontic reasons). The coronal and middle parts of the canals were instrumented using Gates-Glidden no. 3 and 4 drills, and the apical section with the help of Hedstroem size 50-80 files. During instrumentation of the canals irrigation was carried out using 2% chlorhexidine solution. Irrigating solution, volume 2 mL, was introduced into the canal with the use of a syringe and an endodontic needle after using each instrument, and 5 mL volume after complete instrumentation of the canal.
Results:
Observations using a scanning electron microscope with x200 and x1000 magnification showed relatively effective removal of pulp remains and dentine chips, and the presence of a thick smear layer covering the canal walls throughout their length. Statistical analysis did not show significant differences in the degree of cleaning of particular sections of the canals.
Conclusion:
The presence of the smear layer in canals irrigated with chlorhexidine is evidence that it does not have properties for it to be dissolved, and this dictates the necessity of using chelating agents.
Related Concept Videos
Hand hygiene
Hand washing...
Handwashing II: Pre-procedure and Initial Procedure Steps
The hand washing procedure itself includes the following steps. First, cover cuts, if any, on hands with a waterproof dressing. Cuts and abrasions can become contaminated with bacteria hindering the ability to clean the area thoroughly. In addition, repeated hand washing can worsen an injury. The nails must be short and clean, without nail...
Handwashing III: During the Procedure and Post-Procedure Steps

