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Related Experiment Video

Updated: Apr 27, 2026

Shock Wave Application to Cell Cultures

Shock Wave Application to Cell Cultures

Published on: April 8, 2014

Shock waves associated with electric pulses affect cell electro-permeabilization.

Luc Wasungu¹, Flavien Pillet¹, Elizabeth Bellard¹

¹CNRS, IPBS (Institut de Pharmacologie et de Biologie Structurale);205 Route de Narbonne BP64182, F-31077 Toulouse, France; Université de Toulouse, UPS, IPBS, F-31077 Toulouse, France.

Bioelectrochemistry (Amsterdam, Netherlands)

|July 17, 2014

Summary

New applicator designs for cell electro-permeabilization improve safety by using a closed chamber, preventing arcing. However, this design reduced gene expression efficiency due to shock waves.

Keywords:

Electropulsation Electrotransfer High field Pulsing chamber Shock wave

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Area of Science:

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Biotechnology

Background:

High electric field pulses (tens of kV/cm) with short durations (nanoseconds) enable novel cell electro-permeabilization.
Arcing due to air gaps in electrode systems poses a significant safety risk.

Purpose of the Study:

To develop and validate a safe applicator for high-field, short-pulse cell electro-permeabilization.
To investigate the impact of a closed chamber applicator design on electro-mediated permeabilization and electro-gene transfer (EGT).

Main Methods:

A novel closed chamber applicator was designed to mitigate arcing risks.
The safety of the system was validated across millisecond, microsecond, and nanosecond pulse durations.
The applicator's performance in cell electro-permeabilization and EGT was evaluated compared to open chambers.

Main Results:

The closed chamber design successfully delivered high-field, short pulses safely.
A 20-fold decrease in gene expression was observed with the closed chamber compared to open chambers.
Shock waves were detected in the closed chamber configuration, but not in open designs.

Conclusions:

Electropulsation chamber design significantly influences pulsing conditions and EGT efficiency.
The presence of shock waves in the closed chamber design negatively impacts gene electro transfer efficacy.
Further research is needed to optimize closed chamber designs for improved gene transfer outcomes.