[Analysis of components of mesenteric lymph in rats with severe intraperitoneal infection]

To investigate the components of mesenteric lymph of the rats with severe intraperitoneal infection, and inquire into the effect of intestinal lymphatic pathway in severe intraperitoneal infection.

Twenty-four male Wistar rats were randomly divided into two groups according to random number table method, namely model group and sham group with 12 rats in each group. The rat model of severe intraperitoneal infection was reproduced by injecting artificial gastric juice and E.coli intraperitoneally. Mesenteric lymph in both groups was collected 4 hours after the reproduction of the model, and white blood cells were counted and classified. The levels of endotoxin, alkaline phosphatase (AKP), lactate dehydrogenase (LDH), creatine kinase (CK), glutamine transferase (GST), protein and cytokines of mesenteric lymph were determined.

Compared with the sham group, there was an increase in the neutrophil ratio in mesenteric lymph (0.167±0.004 vs. 0.610±0.006, t=33.520, P<0.001), however the percentage of both macrophages (0.009±0.001 vs. 0.020±0.004, t=-6.677, P<0.001) and lymphocytes (0.824±0.005 vs. 0.921±0.004, t=-31.471, P<0.001) was decreased in model group. Compared with sham group, the levels of endotoxin (0.346±0.022 kEU/L vs. 0.186±0.001 kEU/L, t=18.103, P<0.001), AKP [U (king unit): 13.97±5.55 vs. 3.76±0.18, t=4.503, P=0.006], LDH (2 827.45±1 940.32 U/L vs. 712.68±14.09 U/L, t=2.670, P=0.044), CK (2.19±1.21 kU/L vs. 0.70±0.01 kU/L, t=3.035, P=0.029), GST (12.33±6.53 kU/L vs. 1.36±0.39 kU/L, t=4.105, P=0.009) were all significantly elevated. The concentration of protein (4.40±0.48 g/L vs. 2.84±0.16 g/L, t=6.882, P=0.001), tumor necrosis factor-α (TNF-α: 499.39±76.36 ng/L vs. 180.90±70.98 ng/L, t=7.483, P<0.001), interleukin-6 (IL-6: 13.74±0.78 μg/L vs. -0.07±0.07 μg/L, t=52.972, P<0.001), intercellular adhesion molecule-1 (ICAM-1: 2 754.19±221.48 ng/L vs. 1 362.85±393.43 ng/L, t=6.891, P<0.001) and monocyte chemo-attractant protein-1 (MCP-1: 28.23±1.77 μg/L vs. 24.87±1.15 μg/L, t=3.561, P=0.007) and high mobility group protein-1 (HMGB-1: 1 392.78±572.42 ng/L vs. 564.17±21.32 ng/L, t=3.543, P=0.016) in mesenteric lymph in model group were significantly higher than those in sham group.

Intestinal lymphatic pathway maybe the early pathway for the production of remote organ injury caused by severe intraperitoneal infection.