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A constitutive model for a maturing fibrin network.

Thomas H S van Kempen1, Arjen C B Bogaerds2, Gerrit W M Peters3

  • 1Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands.

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|July 17, 2014
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Summary

A new model captures how fibrin networks mature, improving our understanding of blood clot mechanics. This research links structural properties to evolving mechanical behavior, aiding disease study and simulations.

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Area of Science:

  • Biophysics
  • Biomaterials Science
  • Computational Biology

Background:

  • Blood clot formation is vital for hemostasis but implicated in various diseases.
  • The mechanical properties of blood clots are complex, influenced by the fibrin network's structure.
  • Fibrin, the primary structural component, forms a fibrous network that enhances clot rigidity.

Purpose of the Study:

  • To develop a constitutive model for the maturing fibrin network that accounts for evolving mechanical properties.
  • To relate the model's parameters to fundamental structural properties of the fibrin network.
  • To validate the model against experimental data and assess its suitability for simulations.

Main Methods:

  • Developed a constitutive model representing the fibrin network as fibers that thicken over time.
  • Related model parameters (fiber length, bending stiffness, mass-length ratio) to structural properties.
  • Compared model predictions with rheometry experiments tracking network maturation under varying conditions (frequency, fibrinogen concentration).
  • Utilized turbidimetry experiments to determine the mass-length ratio and subsequently derive protofibril number and fiber radius.

Main Results:

  • The model successfully captures the evolving mechanical properties of the maturing fibrin network.
  • Model parameters, particularly the mass-length ratio, correlate well with experimental findings.
  • The mass-length ratio derived from the model aligns with values from turbidimetry, enabling calculation of fiber dimensions.
  • The model demonstrates that mechanical behavior is governed by structural quantities.

Conclusions:

  • The developed constitutive model effectively describes the mechanical properties of maturing fibrin networks based on structural parameters.
  • The model's simplicity makes it suitable for advanced numerical simulations of blood clot formation in physiological contexts, such as blood flow.
  • This work provides a framework for linking microscopic fibrin structure to macroscopic clot mechanics, relevant for both normal physiology and disease states.