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Updated: Apr 26, 2026
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Published on: February 21, 2019
An acridinium-based sensor as a fluorescent photoinduced electron transfer probe for proton detection modulated by
Stefano Basili1, Tiziana Del Giacco, Fausto Elisei
1Dipartimento di Chimica, Biologia e Biotecnologie and Centro di Eccellenza Materiali Innovativi Nanostrutturati (CEMIN), Università di Perugia, Via Elce di sotto 8, 06123, Perugia, Italy. tiziana.delgiacco@unipg.it.
Abstract:
A water-soluble fluorescent pH sensor of 9-amino-10-methylacridinium chromophore with the 2-(diethylamine)ethyl chain as a receptor shows an "off-on" response going from basic to acidic solution. Photoinduced electron transfer has been directly demonstrated to be the quenching mechanism by the observation of the long-lived acridinyl radical. The interaction of the protonated sensor with anionic micelles causes a significant increase in the detection sensitivity of pH.
