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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

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Published on: March 17, 2023

Adipogenic transcriptome profiling using high throughput technologies.

Urmila Basu¹, Josue Moura Romao¹, Le Luo Guan¹

¹Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada.

Journal of Genomics

|July 18, 2014

Summary

This summary is machine-generated.

Recent advances in functional genomics and transcriptomic profiling offer new insights into adipogenesis. This review highlights gene expression and regulatory mechanisms across species using high-throughput

Keywords:

adipogenesis functional genomics gene expression transcriptome

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Area of Science:

Molecular Biology
Genomics
Cell Biology

Background:

Adipogenesis, the process of fat cell formation, is crucial for energy homeostasis.
Understanding the molecular basis of adipogenesis is vital for metabolic research.
Technological advancements are revolutionizing the study of gene expression in adipogenesis.

Purpose of the Study:

To review recent advances in understanding adipogenesis.
To highlight the role of gene expression and regulatory mechanisms.
To showcase the application of high-throughput 'omics' technologies.

Main Methods:

Microarrays
RNA-Sequencing (RNA-Seq)
Chromatin Immunoprecipitation (ChIP)
Next-Generation Sequencing (NGS)
RNA interference (RNAi)
microRNA (miRNA) profiling
Quantitative PCR (qPCR) arrays

Main Results:

High-throughput 'omics' technologies provide deep insights into adipogenesis.
Complex gene expression patterns regulate adipogenesis.
Regulatory mechanisms are conserved across different species.

Conclusions:

Functional genomics and transcriptomics are powerful tools for studying adipogenesis.
Further research using these technologies will elucidate adipogenesis pathways.
Understanding adipogenesis is key to addressing metabolic disorders.