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Asphodosides A-E, anti-MRSA metabolites from Asphodelus microcarpus
Mohammed M Ghoneim1, Khaled M Elokely2, Atef A El-Hela3
1National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA; Department of Pharmacognosy, Faculty of Pharmacy, The University of Al-Azhar, Cairo 11371, Egypt.
Abstract:
Bioassay guided fractionation of the ethanolic extract of Asphodelus microcarpus Salzm. et Viv. (Xanthorrhoeaceae or Asphodelaceae) resulted in isolation of five compounds identified as asphodosides A-E (1-5). Compounds 2-4 showed activity against methicillin resistant Staphylococcus aureus (MRSA) with IC50 values of 1.62, 7.0 and 9.0μg/mL, respectively. They also exhibited activity against Staphylococcus aureus (non-MRSA) with IC50 values of 1.0, 3.4 and 2.2μg/mL, respectively. The structure elucidation of isolated metabolites was carried out using spectroscopic data (1D and 2D NMR), optical rotation and both experimental and calculated electronic circular dichroism (ECD).
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