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Updated: Apr 26, 2026
Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System
Published on: September 20, 2019
Attenuation of migration properties of CD4+ T cells from aged mice correlates with decrease in chemokine receptor
Jihyun Park1, Takuya Miyakawa, Aya Shiokawa
1a Research Center for Food Safety, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
Abstract:
Aging results in attenuation of abilities to mount appropriate immune responses. The influence of aging on CD4(+) T cell migration ability toward chemokines was investigated with young and aged mice. We found functional decline in migration ability toward CCL19 and also decreased CCR7 expression level in antigen-stimulated CD4(+) T cells from aged mice compared with those from young mice. Upon addition of retinoic acid (RA), CD4(+) T cells from aged mice showed decreased CCR9 expression level compared to young mice and the migration ability of CD4(+) T cells from aged mice toward CCL25 was attenuated compared to young mice. We also observed that the expression of RALDH2 mRNA was decreased in mesenteric lymph node dendritic cells from aged mice compared to those from young mice. These results demonstrate that attenuated migration abilities of CD4(+) T cells were observed in aged mice, which correlated with decreased chemokine receptor expression. Furthermore, the reduced production and response to RA by aging may be one of the causes of such attenuated migration abilities in the intestinal immune system.
Insights
Aging impairs CD4(+) T cell migration due to reduced chemokine receptor expression. This decline in immune cell mobility may be linked to aging
Area of Science:
- Immunology
- Aging Research
- Cellular Biology
Background:
- Aging leads to weakened immune responses.
- CD4(+) T cell migration is crucial for effective immunity.
Purpose of the Study:
- To investigate how aging affects CD4(+) T cell migration.
- To identify the role of chemokine receptors and retinoic acid in age-related immune decline.
Main Methods:
- Comparison of CD4(+) T cell migration and chemokine receptor expression in young and aged mice.
- Analysis of retinoic acid (RA) effects on aged CD4(+) T cells.
- Measurement of RALDH2 mRNA expression in dendritic cells.
Main Results:
- Aged mice showed reduced CD4(+) T cell migration towards CCL19 and decreased CCR7 expression.
- Retinoic acid (RA) treatment led to lower CCR9 expression in aged CD4(+) T cells.
- Aged mice exhibited attenuated CD4(+) T cell migration towards CCL25.
- Reduced RALDH2 mRNA expression was observed in aged mesenteric lymph node dendritic cells.
Conclusions:
- Aging attenuates CD4(+) T cell migration, correlating with decreased chemokine receptor expression.
- Reduced production and response to retinoic acid (RA) may contribute to impaired intestinal immunity in aged individuals.
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