Production of wild-type and mutant-type human DAP12 proteins by Bombyx mori nucleopolyhedrovirus vector
Nozomi Uemura1, Hiroko Tabunoki, Taiki Suzuki
1a Department of Bioinformatics and Molecular Neuropathology , Meiji Pharmaceutical University , Tokyo , Japan.
Abstract:
A Japanese patient with Nasu-Hakola disease was found to have a serine-to-asparagine (S39N) substitution in human DNAX-activation protein 12 (DAP12). To elucidate the functional abnormalities of mutant-type DAP12, we expressed wild-type and mutant-type recombinant DAP12 protein with Bombyx mori nucleopolyhedrovirus (BmNPV) vector, and successfully purified the respective proteins from the hemolymph of recombinant BmNPV infected B. mori larvae.
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