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Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

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Staphylococcal cassette chromosome mec elements in methicillin-resistant coagulase-negative staphylococci from a

Vivian Carolina Salgueiro1, Milena Borgo Azevedo, Natalia Lopes Pontes Iorio

  • 1From the *Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Nova Friburgo; †Departamento de Ciência Básica, Universidade Federal Fluminense; and ‡Instituto Municipal da Mulher Fernando Magalhães, Rio de Janeiro, Brazil.

The Pediatric Infectious Disease Journal
|July 19, 2014
PubMed

Insights

This study characterized methicillin-resistant coagulase-negative staphylococci in Brazilian neonates, finding specific clones linked to particular resistance elements. Effective typing aids in understanding the spread of these resistant bacteria.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Neonatal Health

Background:

  • Coagulase-negative staphylococci (CoNS) are significant causes of neonatal infections.
  • Methicillin resistance in CoNS poses a growing public health challenge.
  • Understanding the genetic basis and epidemiology of resistant CoNS is crucial for effective treatment and control.

Purpose of the Study:

  • To characterize methicillin-resistant coagulase-negative staphylococci (MR-CoNS) from Brazilian neonates.
  • To investigate the SCCmec types, antimicrobial susceptibility, and clonality of these isolates.
  • To identify potential associations between specific clones and SCCmec elements.

Main Methods:

  • Molecular characterization of 46 MR-CoNS isolates from Brazilian neonates.
  • Determination of Staphylococcus epidermidis and Staphylococcus haemolyticus species prevalence.
  • SCCmec typing, antimicrobial susceptibility testing, and clonal analysis.

Main Results:

  • Staphylococcus epidermidis and Staphylococcus haemolyticus were the most prevalent species.
  • SCCmec types III or IV were identified in 53.3% of S. epidermidis isolates.
  • A significant proportion (63.6%) of S. haemolyticus isolates were nontypeable for SCCmec.

Conclusions:

  • Specific clones of MR-CoNS were associated with particular SCCmec elements.
  • Effective molecular typing strategies are valuable for epidemiological surveillance of resistant CoNS.
  • Further research is needed to understand the implications of nontypeable SCCmec in S. haemolyticus.

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