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Updated: Apr 26, 2026
08:38
Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
Published on: September 10, 2016
25.4K
[Fermentation transformed ginsenoside by Lactobacillus plantarum]
Summary
Ginseng fermentation using Lactobacillus plantarum enhances total saponins by 32% and increases ginsenoside Rd content. This microbial transformation process is suitable for large-scale production.
Area of Science:
- Microbiology
- Natural Product Chemistry
- Biotechnology
Background:
- Ginseng saponins are valuable compounds with diverse bioactivities.
- Microbial transformation offers a sustainable method for modifying natural products.
- Ginsenoside Rd is a more biologically active metabolite of ginseng saponins.
Purpose of the Study:
- To optimize the fermentation process of ginseng using Lactobacillus plantarum.
- To investigate the microbial transformation of total ginseng saponins into ginsenoside Rd.
- To establish conditions for enhanced production of ginsenoside Rd.
Main Methods:
- Microbial fermentation of ginseng was performed using Lactobacillus plantarum in MRS medium.
- Optimization of fermentation parameters including temperature (35°C) and pH (5.0).
- Quantification of total saponins and ginsenoside Rd using UV spectrophotometry and HPLC, respectively.
Main Results:
- The optimized fermentation process yielded a 32% increase in total saponins.
- A significant increase in ginsenoside Rd content was observed, reaching 4.864 mg/g.
- The fermentation was successfully conducted under static dark culture conditions for 2 days.
Conclusions:
- The developed fermentation process is efficient and suitable for mass production of ginsenoside Rd.
- This study demonstrates the potential of Lactobacillus plantarum for microbial transformation of ginseng saponins.
- The findings hold significance for the biotechnological production of enhanced ginseng metabolites.
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