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Myocardial infarction in rats: effects of metabolic and pharmacologic interventions
H G Zimmer1, P A Martius, G Marschner
1Physiologisches Institut, Universität München, FRG.
Insights
Ribose and Coenzyme Q10 improved heart function after myocardial infarction in rats. These interventions helped restore ATP levels and attenuated negative cardiac changes, offering potential therapeutic benefits for heart attack recovery.
Area of Science:
- Cardiology
- Biochemistry
- Pharmacology
Background:
- Myocardial infarction (MI) significantly impairs left ventricular function and cardiac energy metabolism.
- Understanding the time course of functional and metabolic changes post-MI is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the effects of metabolic and pharmacologic interventions on cardiac function and adenine nucleotide metabolism following induced myocardial infarction in rats.
- To evaluate the therapeutic potential of ribose, gallopamil, and Coenzyme Q10 in mitigating infarct-induced cardiac dysfunction.
Main Methods:
- Myocardial infarction was induced in rats via left coronary artery ligation.
- Cardiac function parameters (LVSP, LV dP/dtmax, LVEDP, cardiac output, stroke volume index) were monitored over nine days.
- Interventions included intravenous ribose, gallopamil, and Coenzyme Q10 administration.
Main Results:
- MI led to progressive decline in left ventricular function and elevated LVEDP.
- ATP content in nonischemic myocardium decreased but recovered spontaneously.
- Ribose attenuated functional decline and promoted ATP restoration; gallopamil worsened function; Coenzyme Q10 improved cardiac output and stroke volume index.
Conclusions:
- Metabolic interventions like ribose show promise in attenuating cardiac dysfunction and restoring energy metabolism post-MI.
- Coenzyme Q10 demonstrated benefits in maintaining circulatory function despite infarct-induced changes.
- Different interventions exhibit distinct effects on infarct-induced cardiac remodeling and function, highlighting targeted therapeutic strategies.
Abstract:
Myocardial infarction was induced in rats by ligation of the descending branch of the left coronary artery. The time course of changes in heart function was recorded within the first nine days. There was a progressive decline in LVSP, in LV dP/dtmax and in the pressure-rate-product. LVEDP was elevated. Cardiac output and stroke volume index were depressed after two days. The ATP content in the nonischemic region was lower than control, but recovered spontaneously toward the normal value within the first four days. Three metabolic and pharmacologic interventions known to affect cardiac adenine nucleotide metabolism were applied. Continuous i.v. administration of ribose which stimulates further adenine nucleotide biosynthesis attenuated the fall and promoted the restoration of ATP in the nonischemic myocardium within four days after coronary artery ligation. The elevation of LVEDP was attenuated with ribose after two and four days. The calcium antagonist gallopamil administered as i.v. infusion for two days led to a further reduction of all parameters of left heart function, but did not influence the increase in adenine nucleotide and protein synthesis that occurred in the nonischemic heart. Coenzyme Q10 had only slight effects on LVSP, LVEDP, and LV dP/dtmax, but attenuated significantly the fall in cardiac output and stroke volume index after two days following coronary artery ligation. Thus, all interventions affected differently the infarct-induced changes in heart and circulatory function. An improvement was observed with ribose and with coenzyme Q10.