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Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Transgenic Rodent Assay for Quantifying Male Germ Cell Mutant Frequency

Published on: August 6, 2014

ENU Mutagenesis in the Mouse.

Rolf Stottmann¹, David Beier²

¹Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.

Current Protocols in Human Genetics

|July 22, 2014

Summary

Researchers treated mice with N-ethyl-N-nitrosourea (ENU) to cause widespread genetic mutations. They also detailed mating strategies to identify mice with observable traits linked to these induced mutations.

Keywords:

alkylating agent genome sequencing per locus mutation frequency phenotype-driven mutagenesis spermatogonial stem cells

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Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

Area of Science:

Genetics
Toxicology
Developmental Biology

Background:

Chemical mutagens are essential tools for genetic research.
N-ethyl-N-nitrosourea (ENU) is a potent mutagen used to induce random point mutations in mammalian genomes.
Understanding mutation induction and detection is crucial for genetic studies.

Purpose of the Study:

To describe the methodology for treating laboratory mice with ENU.
To outline common breeding strategies for identifying ENU-induced mutations.
To facilitate the study of induced mutations and their phenotypic consequences.

Main Methods:

Administration of the mutagen N-ethyl-N-nitrosourea (ENU) to laboratory mice.
Implementation of specific mating schemes (e.g., incross, intercross, outcross) to segregate and identify mutations.
Phenotypic analysis of offspring to detect abnormalities linked to induced mutations.

Main Results:

ENU treatment results in a highly increased rate of mutations across the mouse genome.
Specific mating schemes effectively produce animals that display phenotypes associated with induced mutations.
This approach allows for the efficient screening of mutations.

Conclusions:

ENU is a powerful tool for generating a high frequency of mutations in mice.
The described mating schemes are effective for uncovering mutations with observable phenotypes.
This methodology provides a robust framework for genetic research and the study of gene function.