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Updated: Apr 26, 2026
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
Published on: December 26, 2014
Optimized cell transplantation using adult rag2 mutant zebrafish
Qin Tang1, Nouran S Abdelfattah1, Jessica S Blackburn1
11] Molecular Pathology Unit, Massachusetts General Hospital, Boston, Massachusetts, USA. [2] Cancer Center, Massachusetts General Hospital, Boston, Massachusetts, USA. [3] Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA. [4] Harvard Stem Cell Institute, Cambridge, Massachusetts, USA. [5].
Abstract:
Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) mutant zebrafish are the first immunocompromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.
