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Three-dimensional Imaging and Analysis of Mitochondria within Human Intraepidermal Nerve Fibers
Published on: September 29, 2017
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Intraepidermal nerve fiber analysis using immunofluorescence with and without confocal microscopy
Vincenzo Provitera1, Maria Nolano, Annamaria Stancanelli
1"S. Maugeri" Foundation IRCCS, Center of Telese Terme, Via Bagni Vecchi, 1-82037 Telese Terme, Benevento, Italy.
Muscle & Nerve
|July 22, 2014
Summary
Directly counting intraepidermal nerve fiber (IENF) density using epifluorescence microscopy is as reliable as computer-assisted analysis. This finding simplifies small-fiber neuropathy diagnosis.
Area of Science:
- Neurology
- Histopathology
- Microscopy
Background:
- Intraepidermal nerve fiber (IENF) linear density is crucial for diagnosing small-fiber neuropathies.
- Current methods often involve time-consuming computer-assisted analysis of confocal images.
- Direct counting via epifluorescence microscopy offers an alternative approach.
Purpose of the Study:
- To compare the reliability of direct epifluorescence microscopy counts versus computer-assisted analysis for IENF density.
- To evaluate the agreement between these two distinct methodologies.
Main Methods:
- A comparative study was conducted on 50 skin samples.
- IENF density was measured using both computer-assisted image analysis and direct epifluorescence microscopy.
- Statistical analysis included linear regression and Bland-Altman analysis.
Main Results:
- Excellent agreement was observed between the two methods.
- Linear regression demonstrated a slope close to 1 (β = 0.99).
- Bland-Altman analysis showed a minimal mean difference of 0.46 ± 0.91 fibers/mm.
Conclusions:
- Direct epifluorescence microscopy is a reliable method for quantifying IENF density.
- This technique is as accurate as, but less time-consuming than, 3D computer-assisted analysis.
- Simplifies diagnostic procedures for small-fiber neuropathies.
