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Related Experiment Videos

Heavy hitting: Using water to label humans.

Yonggang Ma1, Andriy Yabluchanskiy, Merry L Lindsey

  • 1San Antonio Cardiovascular Proteomics Center and Mississippi Center for Heart Research, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS, USA.

Proteomics. Clinical Applications
|July 22, 2014
PubMed
Summary

Researchers tracked protein dynamics in healthy humans using deuterium labeling. This method monitors protein turnover rates and aids in clinical trial prognosis and treatment efficacy evaluation.

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Area of Science:

  • Proteomics
  • Biochemistry
  • Clinical Chemistry

Background:

  • Traditional proteomics often relies on static measurements, missing dynamic biological processes.
  • Monitoring protein dynamics, particularly turnover rates, is crucial for understanding physiological and pathological changes in tissues and blood.
  • Deuterium oxide ((2)H2O) labeling offers a valuable approach to quantify protein synthesis and turnover in vivo.

Purpose of the Study:

  • To measure plasma protein turnover dynamics in healthy humans using in vivo (2)H2O labeling.
  • To develop and validate a safe and accessible protocol for (2)H2O administration.
  • To assess the potential of this method for clinical applications, including prognosis and treatment efficacy evaluation.

Main Methods:

  • In vivo administration of deuterium oxide ((2)H2O) to label amino acids.
Keywords:
Deuterium oxideMSMatrix metalloproteinaseProtein

Related Experiment Videos

  • Analysis of (2)H incorporation rates into specific plasma proteins using high-resolution mass spectrometry (MS).
  • Quantification of 542 plasma proteins' turnover dynamics.

    • Main Results:

    • Successfully developed and validated a safe and accessible (2)H2O administration protocol.
    • Measured the in vivo turnover dynamics of 542 plasma proteins in healthy humans.
    • Demonstrated the feasibility of using MS for analyzing (2)H incorporation and calculating protein synthesis rates.

    Conclusions:

    • In vivo (2)H2O labeling is a promising method for evaluating plasma protein dynamics.
    • This technique can provide valuable insights for clinical trials, aiding in prognosis and assessment of treatment efficacy.
    • The study establishes a foundation for broader application of protein turnover analysis in clinical settings.