Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Japanese evidence on Janus kinase inhibitors for rheumatoid arthritis: a narrative review of risk-optimized use.

Frontiers in pharmacology·2026

Same author

Unmasking peripheral T-cell lymphoma in dermatomyositis with anti-PM/Scl-100 and anti-Mi-2 positivity: a case-based review.

Rheumatology international·2026

Same author

Systemic sclerosis with concurrent ANCA-positive IgA nephropathy successfully managed with rituximab and mycophenolate mofetil: a case report.

Immunological medicine·2026

Same author

Association of elevated serum active IL-18 levels with cytokine profiles and clinical features in adult-onset still's disease.

Frontiers in immunology·2026

Same author

Derepression of a Subset of Meiotic Proteins in Primordial Germ Cells of max Mutant Zebrafish.

Development, growth & differentiation·2026

Same author

Metronidazole-induced encephalopathy in a patient with primary Sjögren disease.

Modern rheumatology case reports·2026

Search research articles

Related Experiment Video

Updated: Apr 26, 2026

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Published on: March 3, 2020

Telomere distribution pattern and synapsis initiation during spermatogenesis in zebrafish.

Kenji Saito¹, Chiharu Sakai, Toshihiroa Kawasaki

¹Genetic Strains Research Center, National Institute of Genetics, Mishima, Japan.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|July 22, 2014

Summary

Zebrafish telomeres cluster at the nuclear periphery during meiotic prophase I, forming a bouquet. This telomere clustering is independent of synaptonemal complex formation and can identify meiotic mutants.

Keywords:

Sycp3 meiosis meiotic mutant telomere bouquet

More Related Videos

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Related Experiment Videos

Last Updated: Apr 26, 2026

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Preparation of Meiotic Chromosome Spreads from Zebrafish Spermatocytes

Published on: March 3, 2020

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

Area of Science:

Cell Biology
Genetics
Developmental Biology

Background:

Telomeres, located at chromosome ends, influence chromosomal positioning.
Proper chromosome dynamics are essential for meiosis during spermatogenesis.

Purpose of the Study:

To investigate telomere distribution patterns during zebrafish spermatogenesis.
To analyze telomere behavior, particularly during meiotic prophase I.

Main Methods:

Utilized fluorescence in situ hybridization to visualize telomeres.
Combined with synaptonemal complex protein 3 immunostaining for staging spermatocytes.

Main Results:

Telomeres dispersed in spermatogonia and preleptotene stages.
Formed a 'telomere bouquet' at one nuclear pole during leptotene, coinciding with synapsis initiation.
Spread to the nuclear periphery during zygotene to pachytene stages.
Dispersed again in postmeiotic stages (spermatids and sperm).
Telomere clustering is independent of synaptonemal complex axial element formation.

Conclusions:

Demonstrated distinct telomere clustering and distribution patterns throughout zebrafish spermatogenesis.
The described procedure can be used to screen for meiotic mutants with telomere clustering defects.