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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
Published on: March 21, 2022
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TRIM59 is up-regulated in gastric tumors, promoting ubiquitination and degradation of p53
Zhicheng Zhou1, Zhongzhong Ji2, You Wang2
1State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China.
Gastroenterology
|July 22, 2014
Summary
Tripartite motif 59 (TRIM59) is elevated in gastric tumors and promotes cancer progression by interacting with and degrading P53. This finding offers a potential therapeutic target for gastric cancer.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Gastric carcinogenesis is poorly understood.
- Microarray analysis identified increased messenger RNA (mRNA) expression of tripartite motif 59 (TRIM59) in human gastric tumors compared to normal tissue.
Purpose of the Study:
- To investigate the role of TRIM59 in gastric cancer.
- To explore the mechanism by which TRIM59 affects gastric cancer cell lines and tumor growth.
Main Methods:
- Analyzed microarray data from the Oncomine database.
- Measured TRIM59 mRNA and protein levels in human gastric tissues and cell lines using quantitative polymerase chain reaction and immunoblotting.
- Assessed the effects of TRIM59 knockdown and overexpression on cell proliferation, clone formation, migration, and xenograft tumor growth in mice.
- Examined TRIM59-related signaling pathways and its interaction with P53 and ubiquitin.
Main Results:
- TRIM59 mRNA and protein levels were significantly increased in gastric tumors, correlating with advanced stage and poorer survival.
- TRIM59 knockdown inhibited gastric cancer cell proliferation, migration, and tumor growth, while overexpression had opposite effects.
- TRIM59 physically interacted with P53, promoting its ubiquitination and degradation, leading to reduced expression of P53 target genes.
Conclusions:
- Tripartite motif 59 (TRIM59), a putative ubiquitin ligase, is upregulated in gastric tumors.
- TRIM59 levels correlate with tumor progression and patient survival.
- TRIM59 may promote gastric carcinogenesis by interacting with P53, leading to its ubiquitination and degradation.
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