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Antigen unmasking induced by superheating

Marin Basic1, Maja Jovicic Milentijevic, David Basic

  • 1Faculty of Medicine, University of Nis, Nis, Serbia.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|July 22, 2014
PubMed

This paper is dedicated to the description of superheating as a method for antigen retrieval. In our investigation, this antigen retrieval method was used on thermal plate, heating tissue sections at temperature 120° C for 90 minutes. In the research we conducted the superheating method was applied to formalin-fixed paraffin-embedded tissue sections of breast tumors. The following monoclonal antibodies were used: estrogen receptor, progesterone receptor, epithelial membrane antigen, CD34, and Ki-67. With these tested antibodies we had good staining and no loss of tissue sections during the staining process.

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