Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

International expert consensus recommendations on standardised nomenclature of SSA/Ro (TROVE2/Ro60 and TRIM21/Ro52) autoantibodies in autoimmune diseases.

Annals of the rheumatic diseases·2026
Same author

A New Immunofluorescence Assay Allows the Sensitive Detection of Anti-Cytosolic 5'-Nucleotidase 1A Autoantibodies.

European journal of immunology·2026
Same author

Single-cell multi-omics characterize colorectal tumors, adjacent healthy tissue and matched (tumor) organoids identifying CRC-unique features.

International journal of cancer·2025
Same author

Anti-citrullinated protein antibody detection by hemagglutination.

Rheumatology advances in practice·2025
Same author

Circulating tRNA-derived fragments are decreased in patients with rheumatoid arthritis and increased in patients with psoriatic arthritis.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals·2024
Same author

IL-6 and TNF are Potential Inflammatory Biomarkers in Facioscapulohumeral Muscular Dystrophy.

Journal of neuromuscular diseases·2024

Related Experiment Video

Updated: Apr 26, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

8.9K

Multiplex peptide-based B cell epitope mapping.

Sanne M M Hensen1, Merel Derksen, Ger J M Pruijn

  • 1Department of Biomolecular Chemistry, Radboud Institute for Molecular Life Sciences, Institute for Molecules and Materials and Netherlands Proteomics Centre, Radboud University Nijmegen, 9101, NL-6500 HB, Nijmegen, The Netherlands.

Methods in Molecular Biology (Clifton, N.J.)
|July 23, 2014
PubMed
Summary

This study presents three distinct B cell epitope mapping techniques for identifying antibody-binding sites. These methods enable simultaneous analysis of multiple peptides, aiding in antigen discovery and characterization.

More Related Videos

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

14.5K
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

17.5K

Related Experiment Videos

Last Updated: Apr 26, 2026

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

8.9K
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

14.5K
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
13:58

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

Published on: October 22, 2012

17.5K

Area of Science:

  • Immunology
  • Biochemistry
  • Proteomics

Background:

  • B cell epitope mapping is crucial for understanding antibody-antigen interactions.
  • Accurate epitope identification is vital for vaccine development and diagnostics.

Purpose of the Study:

  • To describe and compare three distinct methods for simultaneous B cell epitope mapping.
  • To provide strategies for epitope identification based on available information about the antigen.

Main Methods:

  • Peptide microarray analysis of overlapping synthetic peptides for linear epitope identification.
  • Random synthetic peptide library on microbeads coupled with microscopy for de novo antigen discovery.
  • Proteolytic digestion of antigen followed by surface plasmon resonance imaging (iSPR) for purified antigens.

Main Results:

  • Each method offers unique advantages depending on the experimental context and prior knowledge of the antigen.
  • Peptide microarrays are effective for established proteins.
  • Microbead libraries and iSPR are versatile for unknown or purified antigens, respectively.

Conclusions:

  • The presented methods offer a comprehensive toolkit for B cell epitope mapping.
  • Method selection should be guided by the availability of the antigenic protein and the desired information.
  • These techniques advance the field of antibody epitope mapping for various applications.