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Hyperthermia inhibited the migration of tongue squamous cell carcinoma through TWIST2

Ya-ling Tang1, Jian Jiang, Jeffrey Liu

  • 1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, (Sichuan University), Chengdu, Sichuan, China; Department of Oral Pathology, West China Hospital of Stomatology, (Sichuan University), Chengdu, Sichuan, China.

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
|July 23, 2014
PubMed
Abstract

Insights

Hyperthermia therapy may reduce tongue squamous cell carcinoma (TSCC) metastasis by lowering TWIST2 expression, a key factor in tumor cell migration. This study explores the link between hyperthermia, TWIST2, and TSCC progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Hyperthermia shows promise for head and neck squamous cell carcinoma (HNSCC) treatment, but its mechanism in reducing tumor metastasis is unclear.
  • TWIST2, a transcription factor crucial for epithelial-mesenchymal transition (EMT), significantly influences tumor progression and metastasis.
  • The specific role of TWIST2 in tongue squamous cell carcinoma (TSCC) and its interaction with hyperthermia remain uninvestigated.

Purpose of the Study:

  • To investigate the role of TWIST2 in TSCC metastasis.
  • To explore the effect of hyperthermia on TWIST2 expression and TSCC cell migration.
  • To elucidate the potential mechanism by which hyperthermia impacts TSCC metastasis.

Main Methods:

  • Immunohistochemical staining assessed TWIST2 expression in 89 TSCC patients.
  • TSCC cell lines were treated with TWIST2-siRNA and subjected to hyperthermia (42.5°C for 40 min) to evaluate migration.
  • Xenograft tumors in nude mice were treated with hyperthermia, and TWIST2 expression was measured.

Main Results:

  • TWIST2 expression correlated with metastasis in human TSCC.
  • TWIST2-siRNA treatment reduced migration in Tca8113 and Cal-27 cells without affecting proliferation or apoptosis.
  • Hyperthermia decreased TWIST2 levels in TSCC and inhibited cell migration.

Conclusions:

  • Hyperthermia may reduce TSCC cell migration by downregulating TWIST2 expression.
  • These findings reveal a novel association between TWIST2 and hyperthermia in TSCC.
  • This research provides insights into hyperthermia's therapeutic potential for TSCC by targeting TWIST2-mediated metastasis.
Keywords:
TWIST2epithelial-mesenchymal transitionhyperthermiamigration

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