UV excitable fluorescence of lumirubin

Solutions of bilirubin in different solvents show negligible fluorescence when they are excited with UV light. After irradiation, blue-violet fluorescence (400-450 nm) is observed, whose intensity and peak position depend on the irradiation time. The isolation of pure lumirubin led us to attribute this fluorescence specifically to this photoisomer, even if small contributions from other photoproducts cannot be ruled out. The emission peak of the pure lumirubin in aqueous solution (phosphate buffer) is at 415 nm while the main excitation peak is at 315 nm. Finally, an interpretation of the observed fluorescence is proposed on the basis of the similarity of the present results with previously reported experimental data.