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Updated: Apr 26, 2026

Metagenomic Analysis of Silage
Published on: January 13, 2017
Effects of microbial additives on chemical composition and fermentation characteristics of barley silage
S M Amanullah1, D H Kim2, H J Lee2
1Division of Applied Life Science (BK21Plus, Institute of Agriculture and Life Science), Gyeongsang National University, Jinju 660-701, Korea ; Bangladesh Livestock Research Institute, Savar, Dhaka-1314, Bangladesh .
Abstract:
This study examined the effects of bacterial inoculants on chemical composition and fermentation indices of barley silage. Barley forage (Youngyang) was harvested at 24% dry matter (DM) and wilted to 47.9% DM. The wilted barley forage was chopped to 3-5 cm length and applied with no inoculant (CON), L. plantarum (1×10(10) cfu/g, LP) or Effective Microorganisms (0.5×10(9) cfu/g, EM). Then the forages were ensiled in four replications for each treatment in 20 L mini silos and stored for 100 days. The contents of crude protein and ether extract were higher in CON silage ensiled for 100-d, while the contents of DM and crude ash were higher in EM silage (p<0.05). The contents of ADF, NDF and hemicellulose as well as the in vitro DM digestibility were not affected by microbial inoculation (p>0.05). The pH, ammonia-N concentration and lactate to acetate ratio were higher (p<0.05) in CON silage, while lactate concentrations were higher (p<0.05) in CON and LP silage. Acetate concentration and lactic acid bacteria was increased (p<0.05) by both inoculants (LP and EM), but propionate concentration and yeast was increased (p<0.05) by EM and LP, respectively. These results indicated that the fermentation quality of barley silage was improved by the application of bacterial inoculants.
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