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[Changes in poly (ADP-ribose) polymerase activity in post-hepatectomized rat liver nuclei with glucagon and insulin]
1Second Department of Surgery, Kinki University School of Medicine, Osakasayama, Japan.
Abstract:
To elucidate the effect of combined therapy of glucagon and insulin on the hepatectomized remnant liver of rats, postoperative changes of liver nuclear poly (ADP-ribose) polymerase activity were measured. The maximal value of poly (ADP-ribose) polymerase activity without glucagon and insulin was revealed as 354 +/- 44pmole/mg/min at the stage of 5 days after partial hepatectomy. In contrast, the enzyme activity with glucagon and insulin reached to peak value as 242 +/- 46pmole/mg/min at the stage of 2 days after hepatectomy. Liver weight regeneration rate of the group with glucagon and insulin showed higher values than that of the group without glucagon and insulin in the period from 5 to 21 days after hepatectomy. The amounts of DNA showed similar changes to poly (ADP-ribose) polymerase activity after hepatectomy. Thus it is suggested that the combined therapy of glucagon and insulin may act to promote liver regeneration through the changes of poly (ADP-ribose) polymerase activity.
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