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Remote Laboratory Management: Respiratory Virus Diagnostics
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A large rodent disease outbreak at a major research facility led to a comprehensive shutdown and decontamination. This effort safeguarded valuable genetically modified mouse lines, offering lessons for future animal health management.

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Area of Science:

  • Laboratory Animal Science
  • Vivarium Management
  • Infectious Disease Outbreak Control

Background:

  • A sentinel monitoring program identified widespread infections, including mouse parvovirus, fur mites, pinworms, and epizootic diarrhea of infant mice, in over 90% of animal colonies.
  • The affected vivarium at the Anschutz Medical Campus supports over 250 principal investigators across a 50,000 net square foot facility.

Purpose of the Study:

  • To detail the planning and execution of a facility-wide shutdown and decontamination process.
  • To describe the rederivation or cryopreservation of over 400 unique genetically modified mouse lines.
  • To analyze the successes and areas for improvement in managing a large-scale rodent disease outbreak.

Main Methods:

  • Enhanced sentinel monitoring program implementation.
  • Facility shutdown and comprehensive decontamination procedures.
  • Rederivation and cryopreservation of genetically modified mouse lines.

Main Results:

  • Over 90% of animal colonies tested positive for multiple infectious agents.
  • Successful rederivation or cryopreservation of more than 400 unique genetically modified mouse lines.
  • Identification of key project successes and areas for future improvement in outbreak management.

Conclusions:

  • A large-scale rodent disease outbreak necessitates a strategic shutdown and decontamination.
  • Preservation of valuable research models, such as genetically modified mouse lines, is critical during such events.
  • Post-outbreak analysis provides valuable insights for refining animal health and facility management protocols.