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Plasma dopa and feeding.

B Banwart1, T D Miller, J D Jones

  • 1Department of Physiology and Biophysics, Mayo Clinic, Rochester, Minnesota 55905.

Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)
|September 1, 1989
PubMed
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Plasma dopa levels remain stable after feeding, supporting its use as a reliable indicator of tyrosine hydroxylase activity. Norepinephrine decreased, while conjugated dopamine increased post-meal.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Physiology

Background:

  • Dopa is a normal plasma constituent in humans and animals.
  • Plasma dopa concentrations are used to assess tyrosine hydroxylase activity, the rate-limiting enzyme in catecholamine synthesis.
  • The impact of feeding on plasma dopa levels is not well understood due to dietary dopa and tyrosine.

Purpose of the Study:

  • To investigate the effect of feeding on plasma dopa and its metabolites in dogs.
  • To determine if plasma dopa can serve as a reliable index of tyrosine hydroxylase activity post-feeding.

Main Methods:

  • Plasma samples from dogs were analyzed for dopa and its major metabolites (3-O-methyldopa, free and conjugated catecholamines) over 5 hours post-feeding.
  • Concentrations of analytes were measured using validated analytical techniques.

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Main Results:

  • Plasma dopa concentrations did not change significantly after feeding.
  • Plasma norepinephrine levels decreased post-feeding.
  • Conjugated dopamine levels increased after feeding.
  • Other measured analytes showed no significant changes.

Conclusions:

  • Plasma dopa is not significantly affected by feeding in dogs.
  • This finding reinforces the utility of plasma dopa as a reliable index for tyrosine hydroxylase activity.
  • Feeding influences other catecholamine metabolites, suggesting complex postprandial neurochemical regulation.