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Detection of calcitonin and CGRP mRNAs in thyroid parafollicular cells using biotinylated probes
Acta Histochemica. Supplementband
|January 1, 1989
Abstract:
Probes for hybridocytochemistry were produced using SP6 RNA polymerase system and biotinylated 11-UTP as a label. Probes were obtained capable of detecting calcitonin-mRNA and CGRP-mRNA. Probe quality was tested using Southern blotting. Hybridocytochemistry on rat thyroid sections demonstrated both mRNAs in all parafollicular cells.