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Updated: Apr 26, 2026
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
Published on: January 2, 2018
Visualizing Notch signaling in vivo in Drosophila tissues
Benjamin E Housden1, Jinghua Li, Sarah J Bray
1Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, NRB 339, 02115, Boston, MA, USA.
Abstract:
The ability to visualize Notch pathway activity in vivo is invaluable for studying the functions and mechanisms of Notch signaling. A variety of tools have been developed to enable monitoring of pathway activity in Drosophila, including endogenous Notch-responsive genes and synthetic transcriptional reporter constructs. Here we summarize some of the different Notch signaling reporters that are available, discuss their relative merits, and describe two methods for visualizing their expression (immunostaining and X-gal staining). These approaches are widely applicable to a range of tissues and stages in Drosophila development.
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