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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Single-cell RNA-seq: advances and future challenges.

Antoine-Emmanuel Saliba1, Alexander J Westermann1, Stanislaw A Gorski1

  • 1Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Straße 2, D-97080 Würzburg, Germany.

Nucleic Acids Research
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Summary
This summary is machine-generated.

Single-cell transcriptomics reveals cell-to-cell variability in molecular composition, impacting biological understanding. This review covers single-cell RNA sequencing (RNA-seq) methods, applications, and future directions in cell biology research.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Phenotypically identical cells exhibit significant behavioral and molecular variations.
  • Cellular heterogeneity is a fundamental aspect of biological systems.
  • Understanding this variation is crucial for basic and clinical research.

Purpose of the Study:

  • To review single-cell transcriptomics techniques, particularly single-cell RNA sequencing (RNA-seq).
  • To highlight the impact of RNA-seq on understanding cell-to-cell variability.
  • To discuss current applications, findings, and future prospects in the field.

Main Methods:

  • Review of various single-cell RNA sequencing (RNA-seq) protocols.
  • Analysis of existing literature on single-cell transcriptomics applications.
  • Synthesis of findings and challenges in the field.

Main Results:

  • Single-cell RNA-seq is a powerful tool for profiling transcriptomic landscapes at the genomic scale.
  • This technology has significantly advanced the understanding of biological processes.
  • Diverse RNA-seq protocols exist, each with specific advantages and limitations.

Conclusions:

  • Single-cell transcriptomics offers unprecedented insights into cellular heterogeneity.
  • Continued development of RNA-seq technologies promises further breakthroughs in biology and medicine.
  • The field is rapidly evolving, addressing complex biological questions.