Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting the distribution of <i>Deyeuxia angustifolia</i> habitats in the Tumen River Basin due to climate and land-use changes.

Frontiers in plant science·2026
Same author

Pretreatment steroids and delayed intervention predict poor long-term outcomes in spinal dural arteriovenous fistulas: a cohort study.

Journal of neurointerventional surgery·2026
Same author

Exploration of potential mechanisms and biomarkers related to ERS-associated RCD in steroid-induced osteonecrosis of the femoral head based on bioinformatics, with experimental validation.

Frontiers in endocrinology·2026
Same author

CT-based interpretable delta-radiomics model for risk stratification of pulmonary ground-glass nodules: a multicentre study.

Insights into imaging·2026
Same author

Clinical outcomes and prognostic factors in patients with spinal dural arteriovenous fistula: a nearly 10-year prospective cohort study.

Journal of neurosurgery. Spine·2026
Same author

Fe<sub>2</sub>O<sub>3</sub>-based nanozyme synergistic with oseltamivir for broad-spectrum inhibition of influenza A virus.

International immunopharmacology·2026

Related Experiment Video

Updated: Apr 26, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

17.5K

Preparation of biofunctionalized quantum dots using microfluidic chips for bioimaging.

Siyi Hu1, Shuwen Zeng, Butian Zhang

  • 1School of Science, Changchun University of Science and Technology, Changchun 130022, Jilin, China. liulw@cust.edu.cn.

The Analyst
|July 24, 2014
PubMed
Summary

This study introduces a novel one-step microfluidic synthesis for protein-functionalized quantum dots (QDs), enhancing efficiency and stability for bioimaging and diagnostics. This method streamlines QD preparation, reducing waste and cost.

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.5K

Related Experiment Videos

Last Updated: Apr 26, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

17.5K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.5K

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Materials Science

Background:

  • Protein-functionalized quantum dots (QDs) are valuable for cellular imaging and sensing.
  • Current QD preparation methods are multi-step, laborious, and costly, hindering clinical applications.
  • A need exists for efficient, scalable QD synthesis for diagnostics.

Purpose of the Study:

  • To develop a one-step synthesis for protein-functionalized QDs using microfluidics.
  • To optimize microfluidic parameters for QD synthesis.
  • To compare the properties of microfluidically synthesized QDs with conventionally prepared ones.

Main Methods:

  • Utilized a microfluidic (MF) chip for one-step synthesis of protein-functionalized QDs.
  • Employed COMSOL simulation modeling to optimize MF reaction parameters (temperature, channel dimensions).
  • Experimentally verified MF synthesis and compared results with conventional bench-top methods using bovine serum albumin (BSA)-functionalized QDs.

Main Results:

  • Achieved a one-step synthesis of protein-functionalized QDs via microfluidics.
  • Optimized MF parameters through simulation and experimental validation.
  • Demonstrated significantly higher protein-functionalization efficiency, photostability, and colloidal stability for MF-synthesized BSA-QDs compared to conventional methods.

Conclusions:

  • The one-step MF approach offers a rapid, cost-effective, and scalable platform for QD formulation.
  • This method reduces chemical waste in QD development.
  • The enhanced properties of MF-synthesized QDs are promising for applications in cell labeling and biomolecular sensing.