Related Experiment Video
Updated: Apr 26, 2026

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Flow cytometric analysis of the physical and protein-binding characteristics of solid drug nanoparticle suspensions
Neill J Liptrott, Marco Giardiello1, Joseph W Hunter1
12Department of Chemistry, The University of Liverpool, Liverpool, UK.
Aim:
Oral and intramuscular sustained-release antiretroviral solid drug nanoparticles (SDNs) are in development but there is limited understanding of whether nanoparticles or dissolved drug predominate systemically.
Materials & Methods:
A flow cytometric method was developed to analyze SDNs in biological fluids such as plasma, including the putative formation of a protein corona.
Results:
SDNs were found to be stable in plasma and could be observed using the techniques developed here. In addition, transferrin, fibrinogen and albumin were found to be associated with SDNs upon incubation.
Conclusion:
This methodology has enabled us to determine protein interactions of SDNs in solution without the requirement of separation from the matrix. This will enable further studies of their biological fate.
More Related Videos
Related Concept Videos
Flow Cytometry
In...
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Factors Affecting Dissolution: Particle Size and Effective Surface Area

