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Updated: Apr 26, 2026

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Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry
Published on: November 8, 2024
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Exploitation of yield stability in barley
Jonathan Mühleisen1, Hans-Peter Piepho, Hans Peter Maurer
1State Plant Breeding Institute, University of Hohenheim, 70593, Stuttgart, Germany, Jonathan.Muehleisen@uni-hohenheim.de.
Summary
Developing hybrid winter barley (Hordeum vulgare L.) varieties, instead of line varieties, can enhance grain yield and stability. This is crucial for adapting to extreme weather conditions and improving crop resilience.
Area of Science:
- Agronomy
- Plant Breeding
- Genetics
Background:
- Yield stability is crucial for winter barley (Hordeum vulgare L.) to adapt to increasing extreme weather events.
- Developing high-yielding and stable varieties is a key objective in barley breeding programs.
- Current breeding strategies may not adequately capture yield stability due to trial limitations.
Purpose of the Study:
- To determine the optimal field trial size for accurately assessing winter barley genotype yield stability.
- To compare the grain yield performance and stability of two-rowed and six-rowed lines with hybrids.
- To investigate the relationship between agronomic traits and yield stability in winter barley.
Main Methods:
- Analysis of five series of 3-year winter barley registration trials in Germany.
- Evaluation of static and dynamic yield stability and grain yield performance across 10-45 environments.
- Comparison of six-rowed hybrids, six-rowed inbred lines, and two-rowed inbred lines.
Main Results:
- Precise yield stability assessment requires phenotyping in at least 40 environments, exceeding typical breeding program capacity.
- No consistent association was found between agronomic traits and yield stability, limiting indirect selection.
- Hybrids demonstrated superior grain yield performance and dynamic yield stability compared to line varieties.
Conclusions:
- Breeding hybrid winter barley varieties offers a promising strategy for achieving both high yield and enhanced yield stability.
- The development of hybrid varieties is essential for future-proofing barley crops against climate variability.
- Current field trial dimensions may be insufficient for robust yield stability selection.

