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Updated: Apr 26, 2026

Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
Published on: March 12, 2015
Label-free electrochemiluminescent enantioselective sensor for distinguishing between chiral metallosupramolecular
Li Wu1, Chuanqi Zhao, Jinsong Ren
1Laboratory of Chemical Biology, Division of Biological Inorganic Chemistry, State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022 (R.P. China); University of the Chinese Academy of Science, Beijing, 100039 (R.P. China).
Abstract:
Chiral molecular recognition of DNA is important for rational drug design and for developing structural probes of DNA conformation. Developing a convenient and inexpensive assay for sensitive and selective identification of DNA-specific binding compounds with rapid, easy manipulation is in ever-increasing demand. Here, we present a "turn-on" and label-free electrochemiluminescent (ECL) biosensor for distinguishing chiral metallosupramolecular complexes based on DNA three-way junction formation selectively induced by the analyte. The fabricated ECL sensor shows excellent performance in the chiral discrimination of two enantiomers with an enantioselective recognition ratio of up to 4.4. More importantly, as a "turn-on" detection system, the ECL chiral sensor does not suffer from false positives and limited signal range of "signal-off" systems. Therefore, this concept may provide a new insight into the design of efficient sensors for distinguishing chiral molecules and for investigating the interactions between DNA and small molecules.
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