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Published on: August 2, 2011
A complete molecular diagnostic procedure for applications in surveillance and subtyping of avian influenza virus
Chun-Hsien Tseng1, Hsiang-Jung Tsai2, Chung-Ming Chang3
1Animal Health Research Institute, Danshui, Taipei 25158, Taiwan.
Introduction:
The following complete molecular diagnostic procedure we developed, based on real-time quantitative PCR and traditional PCR, is effective for avian influenza surveillance, virus subtyping, and viral genome sequencing.
Method:
This study provides a specific and sensitive step-by-step procedure for efficient avian influenza identification of 16 hemagglutinin and 9 neuraminidase avian influenza subtypes.
Result And Conclusion:
This diagnostic procedure may prove exceedingly useful for virological and ecological advancements in global avian influenza research.

