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Cytoplasmic pH in cultured porcine thyroid cells: alkalinization by Ca2+
N Takasu1, I Komiya, Y Nagasawa
1Department of Gerontology, School of Medicine, Shinshu University, Nagano-ken, Japan.
Journal of Molecular Endocrinology
|September 1, 1989
Abstract:
The role of calcium in cytoplasmic pH (pHi) changes was studied using 2',7'-bis(2-carboxyethyl)-5-(and 6-)carboxyfluorescein, an internalized fluorescent pH indicator, in cultured porcine thyroid cells. The Ca2+ ionophores A23187 and ionomycin stimulated thyroid cell alkalinization. An increase in cytoplasmic free calcium resulted in activation of Na+/H+ exchange which alkalinized the cells.