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Updated: Apr 26, 2026

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
Published on: June 30, 2016
A new method for rapid construction of a Pseudomonas sp. HF-1 bioaugmented system: accelerating acylated homoserine
Mei-Zhen Wang1, Xin Zheng2, Kun Zhang2
1School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China; Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, Hangzhou 310012, China.
Abstract:
Pseudomonas sp. HF-1 bioaugmented systems were operated to treat tobacco wastewater under pH 5.5 for three cycles and pH 8.0 for the rest, which was suitable for HF-1 biofilm formation. The results showed that, under pH control, the contents of 3-oxo-C6-HSL, C6-HSL and 3-oxo-C8-HSL were significantly higher than HF-1 thresholds for biofilm formation. Compared with non-pH controlled reactors, HF-1 showed greater colonization in pH controlled reactors, primarily owing to the high extracellular polymeric substances secretion induced by quorum sensing. Accordingly, high indigenous community activity and granular sludge were observed. Sludge granulation occurred from the seventh cycle, and the average diameter was greater than 400 μm. These systems were also highly efficient with nearly 100% nicotine degradation and 60% total organic carbon removal. Overall, the results indicate that pH regulation is a new and feasible method for acceleration of releasing of auto-inducers, which is beneficial to construction of HF-1 bioaugmented systems.
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