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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Related Experiment Video

Updated: Apr 26, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes
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Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

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Generating genetically modified mice using CRISPR/Cas-mediated genome engineering.

Hui Yang1, Haoyi Wang1, Rudolf Jaenisch2

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.

Nature Protocols
|July 25, 2014
PubMed
Summary
This summary is machine-generated.

This study presents a rapid CRISPR/Cas genome engineering protocol for creating gene-modified mice in just 4 weeks. This method enables simultaneous editing of multiple genes, accelerating research in development and disease.

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Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Developmental Biology

Background:

  • * Traditional gene targeting in mice using embryonic stem (ES) cells is effective but complex and time-consuming.
  • * CRISPR/Cas technology offers a more efficient alternative for genetic modification.
  • * Gene-modified mice are crucial for studying biological processes and diseases.

Purpose of the Study:

  • * To provide a detailed protocol for generating gene-modified mice using CRISPR/Cas technology.
  • * To demonstrate the efficiency of CRISPR/Cas for simultaneous multi-gene editing.
  • * To establish a rapid method for creating genetically engineered mouse models.

Main Methods:

  • * CRISPR/Cas-mediated genome engineering.
  • * Piezo-driven injection of nucleic acids into mouse embryos.
  • * Simultaneous editing of multiple genes (five or more) in ES cells.

Main Results:

  • * Generation of gene-modified mice in as little as 4 weeks from target design.
  • * Successful simultaneous editing of multiple genes using CRISPR/Cas technology.
  • * Demonstrated applicability of the protocol in rats and primates.

Conclusions:

  • * CRISPR/Cas technology significantly accelerates the generation of gene-modified mice.
  • * The protocol facilitates sophisticated genome engineering, including multi-gene editing.
  • * This approach is adaptable for species lacking established ES cell lines.