Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Replisome03:01

The Replisome

31.1K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
31.1K
The Replisome03:01

The Replisome

9.7K
9.7K
The DNA Replication Fork01:02

The DNA Replication Fork

30.5K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
30.5K
The DNA Replication Fork01:02

The DNA Replication Fork

17.9K
17.9K
Replication in Prokaryotes02:35

Replication in Prokaryotes

86.4K
Overview
86.4K
Replication in Prokaryotes01:32

Replication in Prokaryotes

20.3K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
20.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A conserved mechanism for dimerization and activation of superfamily 1A UvrD-family helicases.

Nucleic acids research·2026
Same author

Health outcomes of socially vulnerable populations in different Medicare payment arrangements.

The American journal of managed care·2026
Same author

Dissecting the role of PCNA and Pif1 in replication of individual DNA molecules by DNA polymerase δ.

bioRxiv : the preprint server for biology·2026
Same author

A Conserved Mechanism for Dimerization and Activation of Superfamily 1A UvrD-family Helicases.

bioRxiv : the preprint server for biology·2026
Same author

Regulation of Pfh1 helicase activity by nucleic acid interactions and mitochondrial SSB.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

3D-Printing-Assisted, Microfabricated Devices Reveal Hierarchical and Temporal Mechanosensing in High-Density Fibroblast Culture.

ACS nano·2026

Related Experiment Video

Updated: Apr 26, 2026

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

1.9K

Diffusion of human replication protein A along single-stranded DNA.

Binh Nguyen1, Joshua Sokoloski1, Roberto Galletto1

  • 1Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Journal of Molecular Biology
|July 25, 2014
PubMed
Summary

Human replication protein A (hRPA) rapidly diffuses along single-stranded DNA (ssDNA), facilitating genome maintenance. This dynamic movement allows hRPA to disrupt DNA hairpins and enable access for other proteins.

Keywords:
DNA hairpin meltingRPAdiffusion coefficientdynamicssingle-molecule fluorescence

More Related Videos

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.3K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Related Experiment Videos

Last Updated: Apr 26, 2026

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

1.9K
Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion
05:22

Author Spotlight: Characterizing DNA Replication of Pathogenic Repeats to Uncover Mechanisms of Replication Fork Stalling and Expansion

Published on: September 13, 2024

1.3K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Replication protein A (RPA) is a crucial eukaryotic protein that binds single-stranded DNA (ssDNA).
  • RPA is essential for DNA replication, recombination, and repair, but its dynamic interactions with ssDNA are not fully understood.
  • RPA must be removed or redistributed to allow other proteins access to ssDNA during genome maintenance.

Purpose of the Study:

  • To investigate the dynamics of human RPA (hRPA) interactions with ssDNA.
  • To determine if hRPA can diffuse along ssDNA and the functional implications of this diffusion.

Main Methods:

  • Combined ensemble and single-molecule fluorescence approaches.
  • Utilized a Cy3 fluorophore attached to ssDNA to monitor hRPA position.
  • Developed a novel calibration method to quantify hRPA diffusion on ssDNA.

Main Results:

  • Demonstrated that hRPA heterotrimers can rapidly diffuse along ssDNA.
  • Showed that hRPA diffusion is functional, enabling transient disruption of DNA hairpins.
  • Estimated a one-dimensional diffusion coefficient for hRPA on ssDNA of approximately 5000 nt(2)/s at 37°C.

Conclusions:

  • hRPA exhibits rapid 1D diffusion along ssDNA, a key mechanism for its function.
  • This diffusion allows hRPA to reposition, facilitating access for other DNA-binding proteins.
  • Understanding hRPA dynamics provides insights into efficient genome maintenance processes.