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Updated: Aug 9, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
[An identification of special antibodies for brucellosis]
Abstract:
Applying antigens of B. abortus and Y. enterocolitica 0:3, detection 88 sera of cattle in foci of Brucellosis was carried out by Dot-ELISA on nitrocellulose filter paper, and compared with conventional ELISA and SAT. The results showed that coincident rate of Dot-ELISA and ELISA was 98%. Their sensitivity was more than SAT. In contrast with others, the Dot-ELISA should differentiate Brucella antibody from antibody to Y. enter. 0:9. In addition, the method is a simple, Speed, stable, and very easy. The procedure should help clinical diagnosis and epidemiological investigation.

