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Sox4 up-regulates Cyr61 expression in colon cancer cells

Gang Wu1, Yuan-Zeng Zhu, Jian-Cheng Zhang

  • 1Department of General Surgery, Henan Provincial People' s Hospital, People's Hospital of Zhengzhou University, Zhengzhou, People' s Republic of China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|July 26, 2014
PubMed
Abstract

Insights

This study reveals a new molecular network involving Sox4 and Cyr61 in colon cancer. This pathway may be key to controlling colon cancer cell growth and survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant oncogene activation is critical in colon cancer development.
  • Sox4, a transcription factor, is implicated in tumorigenesis.

Purpose of the Study:

  • To identify transcriptional targets of Sox4.
  • To elucidate the role of Sox4 in colon cancer.

Main Methods:

  • PCR-based microarrays to identify Sox4 targets.
  • Small interfering RNA (siRNA) for Sox4 knockdown.
  • Luciferase and chromatin immunoprecipitation (ChIP) assays for transcriptional regulation analysis.

Main Results:

  • Cyr61, an extracellular matrix protein, was identified as a transcriptional target of Sox4.
  • Sox4 overexpression elevated Cyr61 expression; Sox4 knockdown reduced it.
  • A Sox4 binding motif was found in the Cyr61 promoter.

Conclusions:

  • A novel Sox4-Cyr61 molecular network was identified.
  • This network potentially regulates colon cancer cell proliferation and survival.

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