Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunochemical assays for IgG, IgA, IgM and transferrin compared.

R H Christenson1, P R Finley, L M Silverman

  • 1Department of Laboratory Service, Durham VA Medical Center, NC 27705.

Clinical Biochemistry
|August 1, 1989
PubMed
Summary

This study compared immunochemical methods for quantifying IgG, IgA, IgM, and transferrin. The Abbott TDx showed good agreement for IgA and transferrin but discrepancies for high IgM levels compared to other methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trial design for assessing analytical and clinical performance of high-sensitivity cardiac troponin I assays in the United States: The HIGH-US study.

Contemporary clinical trials communications·2019
Same author

Effects of dietary carbohydrate on 1,5-anhydroglucitol in a population without diabetes: results from the OmniCarb trial.

Diabetic medicine : a journal of the British Diabetic Association·2017
Same author

Recommendation to harmonize the units for reporting cardiac troponin results.

Clinica chimica acta; international journal of clinical chemistry·2013
Same author

[The ways of harmonization of clinical laboratory measurement techniques].

Klinicheskaia laboratornaia diagnostika·2013
Same author

Nonlinear restoration of noisy images.

IEEE transactions on pattern analysis and machine intelligence·2011
Same author

A CFTR mutation (D1152H) in a family with mild lung disease and normal sweat chlorides.

Clinical genetics·2005

Area of Science:

  • Clinical Chemistry
  • Immunology
  • Biotechnology

Background:

  • Accurate quantification of serum proteins like immunoglobulins (IgG, IgA, IgM) and transferrin is crucial for diagnosing various medical conditions.
  • Evaluating new immunochemical methods against established techniques is essential for clinical laboratory practice.

Purpose of the Study:

  • To compare the performance of different immunochemical methods for determining IgG, IgA, IgM, and transferrin in serum.
  • To assess a newly developed nephelometric method (Abbott TDx) against existing methods like radial immunodiffusion (RID) and rate nephelometry (Beckman ICS).

Main Methods:

  • Comparative analysis of immunochemical methods: radial immunodiffusion (RID), Beckman ICS (rate nephelometry), Abbott TDx (nephelometry), and immunoturbidimetry.
  • Studies included calibrator crossover, patient sample comparison, and determination of normal reference intervals.

Related Experiment Videos

Main Results:

  • Good agreement was observed for IgA and transferrin quantification between methods.
  • Acceptable agreement was found between Beckman ICS and Abbott TDx for IgG, but neither agreed with RID.
  • Discrepancies were noted for high IgM levels on the Abbott TDx compared to other methods.
  • Normal reference intervals determined on the Abbott TDx aligned well with other methods.

Conclusions:

  • The Abbott TDx demonstrates good performance for IgA, transferrin, and IgG (compared to ICS).
  • Further validation is needed for high IgM quantification on the Abbott TDx.
  • The new nephelometric method shows promise, with established reference intervals comparable to existing techniques.