Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 26, 2026

Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography
02:42

Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography

Published on: January 17, 2025

878

Surface landmark quantification of embryonic mouse craniofacial morphogenesis.

Christopher J Percival, Rebecca Green, Ralph Marcucio

  • 1Department of Cell Biology and Anatomy, Alberta Children's Hospital Institute for Child and Maternal Health, The McCaig Bone and Joint Institute, University of Calgary, Calgary, AB T2N 4N1, Canada. bhallgri@ucalgary.ca.

BMC Developmental Biology
|July 26, 2014
PubMed
Summary

A standardized set of craniofacial landmarks for embryonic mice (E10.5-12.5) aids in studying developmental variations. Removing high-error landmarks ensures reliable data for comparative analyses of craniofacial morphogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revisiting the Palimpsest: Exploring the Interaction of the Variance and Structure of Developmental Determinants of Phenotypic Covariance.

Integrative and comparative biology·2026
Same author

Meet the editorial team. An interview with Ralph Marcucio, Assistant Editor, University of California San Francisco, United States.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same author

Three-dimensional cellular dynamics and mandibular morphogenesis.

Frontiers in cell and developmental biology·2026
Same author

Balanced Fluid or 0.9% Saline in Children Treated for Septic Shock.

The New England journal of medicine·2026
Same author

Using end-to-end attribution to link greenhouse gas emitters to child health outcomes.

Archives of disease in childhood·2026
Same author

Age-related Delays in Osteochondral Remodeling of Fracture Healing Illustrated by Mass Spectrometry Imaging.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Developmental Biology
  • Morphometrics
  • Genetics

Background:

  • Subtle craniofacial variations in embryonic mice are key to understanding developmental perturbations.
  • Comparing landmark coordinate data across mouse models enhances knowledge of craniofacial variation.
  • A standardized set of craniofacial surface landmarks for embryonic day (E) 10.5-12.5 mice is proposed.

Purpose of the Study:

  • To establish a standard set of craniofacial surface landmarks for embryonic mice (E10.5-12.5).
  • To quantify intra- and inter-observer landmark placement variation.
  • To assess the impact of landmark set selection on ontogenetic analyses.

Main Methods:

  • Quantification of intra- and inter-observer landmark placement error for a proposed standard set of craniofacial landmarks.

More Related Videos

Quantification of Orofacial Phenotypes in Xenopus
09:26

Quantification of Orofacial Phenotypes in Xenopus

Published on: November 6, 2014

9.2K
Midface Hypoplasia and Cranial Base Morphology in Syndromic Craniosynostosis: A Comparative Analysis Study Using a Predictive Regression Model
08:03

Midface Hypoplasia and Cranial Base Morphology in Syndromic Craniosynostosis: A Comparative Analysis Study Using a Predictive Regression Model

Published on: November 4, 2025

445

Related Experiment Videos

Last Updated: Apr 26, 2026

Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography
02:42

Analysis of Craniomaxillofacial Malformations in Mice Using Three-dimensional Microcomputed Tomography

Published on: January 17, 2025

878
Quantification of Orofacial Phenotypes in Xenopus
09:26

Quantification of Orofacial Phenotypes in Xenopus

Published on: November 6, 2014

9.2K
Midface Hypoplasia and Cranial Base Morphology in Syndromic Craniosynostosis: A Comparative Analysis Study Using a Predictive Regression Model
08:03

Midface Hypoplasia and Cranial Base Morphology in Syndromic Craniosynostosis: A Comparative Analysis Study Using a Predictive Regression Model

Published on: November 4, 2025

445
  • Identification of landmarks with the highest placement error.
  • Analysis of ontogenetic shape changes with and without high-error landmarks.

    • Main Results:

    • Intraobserver error generally stayed below 0.1 mm, with some landmarks showing higher error at E11.5 and E12.5.
    • Interobserver error increased with embryonic age, particularly for landmarks on wide curves or facial processes.
    • Removing high-error landmarks did not significantly alter the vectors of craniofacial shape change in ontogenetic regression.

    Conclusions:

    • A single observer is preferred for landmark identification within a study.
    • Significant training is required for landmarkers contributing to meta-analyses.
    • The proposed standard landmark set, with high-error landmarks removed, can form a basis for comparative studies of facial morphogenesis and phenotypic variation.