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Updated: Apr 26, 2026

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice
Published on: July 16, 2014
Synergic effects of pregabalin-acetaminophen combination in somatic and visceral nociceptive reactivity
Liliana Mititelu Tartau1, Eliza Gratiela Popa, Raoul Vasile Lupusoru
1Department of Pharmacology-Algesiology, University of Medicine and Pharmacy 'Grigore T. Popa', Iasi, Romania.
Background/Aims:
The present study investigates the effects of pregabalin (PGB), acetaminophen (ACET) and tenoxicam (TNX) administration in somatic and visceral nociception, using the tail flick test and the writhing test in mice.
Methods:
In the tail flick test, the substances were administered orally and the latency time response was recorded 15, 30, 60, 90 and 120 min after administration. In the writhing test, pain responses were scored every 5 min during a 30-min period after intraperitoneal injection of diluted acetic acid.
Results:
Our study demonstrated that oral administration of the combination PGB-ACET resulted in a stronger increase of latency reaction - statistically significant after 15 min compared to TNX and after 30 min compared to PGB in tail flick test. In the writhing test, the combination PGB-ACET, but also PGB-TNX, resulted in a stronger decrease of writhe numbers - statistically significant compared to the effects of the separate administration of each substance. This decrease was more intense in animals treated with the combination PGB-ACET than with PGB-TNX.
Conclusion:
These results suggest an antinociceptive activity which may be a consequence of the synergic action of the substances.
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