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Published on: April 29, 2016
TLR7-expressing cells comprise an interfollicular epidermal stem cell population in murine epidermis
Chaoran Yin1, Ting Zhang1, Liangjun Qiao2
11] Laboratory of Stem Cell Biology, Center for Medical Stem Cell Biology, State Key Laboratory of Biotherapy, Ministry of Health, West China Hospital, Sichuan University, Chengdu, China, 610041 [2].
Abstract:
Normal interfollicular epidermis (IFE) homeostasis is maintained throughout the entire life by its own stem cells that self-renew and generate progeny that undergo terminal differentiation. However, the fine markers of the stem cells in interfollicular epidermis are not well defined yet. Here we found that TLR7 identified the existence of progenitors and interfollicular epidermal stem cells in murine skin. In vitro, TLR7-expressing cells comprised of two subpopulations that were competent to proliferate and exhibited distinct differentiation potentials. Three-dimensional (3D) organotypic culture and skin reconstitution assays showed that TLR7-expressing cells were able to reconstruct the interfollicular epidermis. Finally, TLR7-expressing cells maintained the intact interfollicular epidermal structures revealed in serial transplantation assays in vivo in mice. Taken together, our results suggest that TLR7-expressing cells comprise an interfollicular epidermal stem cell population.
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