Atmospheric scanning electron microscope system with an open sample chamber: configuration and applications

Area of Science:

• Materials Science
• Nanotechnology
• Cell Biology

Background:

• Correlative microscopy requires aligning optical and electron imaging modalities.
• Observing dynamic processes in liquids necessitates specialized microscopy setups.

Purpose of the Study:

• To describe and report recent developments of an atmospheric scanning electron microscope (ASEM) with an open sample chamber and optical microscope (OM).
• To enable quasi-simultaneous correlative microscopy of liquid-immersed samples.
• To demonstrate the system's capability for in-situ dynamic observations and chemical/biological experiments.

Main Methods:

• Development of the ClairScope system, integrating an inverted SEM with an OM for aligned, simultaneous imaging.
• Utilizing an open sample chamber allowing liquid immersion and reagent administration.
• Implementing a motorized stage, column protection, and a GUI for OM/SEM operation.

Main Results:

• Demonstrated cathodoluminescence of ZnO particles in solution.
• Observed the influence of NaCl on silica particle motion.
• Examined Varp's effect on Tyrp1 trafficking in melanocytes using fluorescence.
• Dynamically observed colloidal silver nanoparticle sintering up to 240°C.

Conclusions:

• The enhanced ASEM system facilitates correlative microscopy in solution for diverse applications.
• The open chamber design supports in-situ manipulation and dynamic observations.
• The ClairScope system advances the study of nanoscale phenomena and biological processes in liquid environments.