The hematopoietic stem cell landscape

Hematopoietic stem cells (HSCs) play critical roles in regulating normal blood cell development. Although initially these cells were mysterious and difficult to study in isolation, those obstacles have progressively been rolled away in just a few decades to reveal a heterogeneity of repopulating activity, cell proliferation, and energy metabolism within defined stem cell populations based on drug transporter and cell surface marker expression. A wide range of new technologies have driven innovative discovery of the regulators of HSCs and continued to move the field forward toward a full view of the landscape of single HSCs at the gene and protein levels. It is the goal of this overview chapter to summarize the array of techniques included in the third edition of Hematopoietic Stem Cell Protocols which will aid investigators in the field.