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Related Concept Videos

Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

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The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
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Hematopoiesis01:21

Hematopoiesis

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The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
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Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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Overview of Hematopoiesis01:20

Overview of Hematopoiesis

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Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Stem Cell Niche01:26

Stem Cell Niche

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The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
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Related Experiment Video

Updated: Apr 26, 2026

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
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The hematopoietic stem cell landscape.

Kevin D Bunting1, Cheng-Kui Qu

  • 1Department of Pediatrics, Aflac Cancer and Blood Disorders Center, Emory University School of Medicine, 1760 Haygood Drive NE, HSRB E308, Atlanta, GA, 30322, USA, kevin.bunting@emory.edu.

Methods in Molecular Biology (Clifton, N.J.)
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Hematopoietic stem cells (HSCs) are crucial for blood development. New technologies reveal their diverse activities, aiding researchers in studying these vital cells at the gene and protein levels.

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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
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Area of Science:

  • Hematology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Hematopoietic stem cells (HSCs) are essential for blood cell formation.
  • Historically, studying HSCs in isolation was challenging.
  • Recent advancements have uncovered heterogeneity in HSC function.

Purpose of the Study:

  • To summarize techniques for studying hematopoietic stem cells.
  • To provide a resource for investigators in the field.
  • To highlight advancements in understanding HSC heterogeneity.

Main Methods:

  • Utilizing drug transporter and cell surface marker expression for HSC definition.
  • Employing a wide array of new technologies for discovery.
  • Analyzing gene and protein expression at the single HSC level.

Main Results:

  • Revealed heterogeneity in HSC repopulating activity, proliferation, and metabolism.
  • Identified regulators of HSC function through innovative technologies.
  • Enabled a comprehensive view of single HSCs.

Conclusions:

  • Technological advancements have overcome previous obstacles in HSC research.
  • Understanding HSC heterogeneity is key to advancing the field.
  • This overview provides essential protocols for continued investigation.