The S28H mutation on mNeptune generates a brighter near-infrared monomeric fluorescent protein with improved quantum
Zhao-Yang Li1, Dian-Bing Wang2, Zhi-Ping Zhang3
1State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
For living deep-tissue imaging, the optical window favorable for light penetration is in near-infrared wavelengths, which requires fluorescent proteins with emission spectra in the near-infrared region. Here, we report that a single mutant Ser28His of mNeptune with a near-infrared (≥650 nm) emission maxima of 652 nm is found to improve the brightness, photostability, and pH stability when compared with its parental protein mNeptune, while it remains as a monomer, demonstrating that there is still plenty of room to improve the performance of the existing near infrared fluorescence proteins by directed evolution.
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