Related Experiment Video

Updated: Apr 26, 2026

Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
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Identification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR

Published on: February 1, 2013

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Genetically engineered insertional mutagenesis in mice to model cancer: Sleeping Beauty

Viive M Howell1, Emily K Colvin

  • 1Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research, Royal North Shore Hospital, University of Sydney, Level 8, Kolling Building, St Leonards, NSW, 2065, Australia, viive.howell@sydney.edu.au.

Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2014
PubMed

Insights

The Sleeping Beauty system creates advanced mouse cancer models for studying cancer development and testing new treatments. This review covers existing Sleeping Beauty cancer models and strategies for generating new ones.

Area of Science:

  • Oncology
  • Genetics
  • Animal Models

Background:

  • Accurate human cancer models in mice are crucial for understanding cancer biology and preclinical drug testing.
  • The Sleeping Beauty (SB) transposon system has revolutionized the creation of genetically engineered mouse models (GEMMs).

Purpose of the Study:

  • To review existing cancer models developed using the Sleeping Beauty system.
  • To outline strategies for generating novel cancer models with the SB system.

Main Methods:

  • Utilizing insertional mutagenesis via the Sleeping Beauty system in mice.
  • Leveraging available SB mouse strains and genetic engineering techniques.

Main Results:

  • The SB system has enabled the development of a new generation of GEMMs for cancer research.
  • Novel cancer-causing genes have been identified through SB-based insertional mutagenesis studies.

Conclusions:

  • The Sleeping Beauty system provides a powerful platform for in vivo cancer research.
  • This review serves as a guide for researchers utilizing SB to create and study diverse cancer models.

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